Selective sensing of pyrophosphate in physiological media using zinc(II)dipicolylamino-functionalised peptides
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Open Access
Type
ArticleAbstract
A series of linear peptide based anion receptors, in which the distance between the bis[zinc(II)dipicolylamine] binding sites and the peptide backbone was varied systematically, was prepared and their anion binding ability was investigated using indicator displacement assays. ...
See moreA series of linear peptide based anion receptors, in which the distance between the bis[zinc(II)dipicolylamine] binding sites and the peptide backbone was varied systematically, was prepared and their anion binding ability was investigated using indicator displacement assays. Shortening the distance between the binding site and the peptide backbone was found to enhance both the receptor affinity and selectivity for pyrophosphate over other organic polyphosphate anions in Krebs buffer with the maximum selectivity and affinity observed with a spacer length of two methylene units. The suitability of these receptors for the determination of pyrophosphate concentrations in Krebs buffer and in artificial urine was examined.
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See moreA series of linear peptide based anion receptors, in which the distance between the bis[zinc(II)dipicolylamine] binding sites and the peptide backbone was varied systematically, was prepared and their anion binding ability was investigated using indicator displacement assays. Shortening the distance between the binding site and the peptide backbone was found to enhance both the receptor affinity and selectivity for pyrophosphate over other organic polyphosphate anions in Krebs buffer with the maximum selectivity and affinity observed with a spacer length of two methylene units. The suitability of these receptors for the determination of pyrophosphate concentrations in Krebs buffer and in artificial urine was examined.
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Date
2015-01-01Publisher
Royal Society of ChemistryCitation
Zwicker, V. E., Long, B. M., & Jolliffe, K. A. (2015). Selective sensing of pyrophosphate in physiological media using zinc(ii)dipicolylamino-functionalised peptides. Organic & Biomolecular Chemistry, 13(28), 7822–7829. https://doi.org/10.1039/c5ob00956aShare