A fluorogenic probe for cell surface phosphatidylserine using an intramolecular indicator displacement sensing mechanism

Zwicker, Vincent E.; Olivera, Bruno L.; Yeo, Jia Hao; Fraser, Stuart T.; New, Elizabeth J.; Bernardes, Goncalo J. L.; Jolliffe, Katrina A.

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Open Access

Field	Value	Language
dc.contributor.author	Zwicker, Vincent E.
dc.contributor.author	Olivera, Bruno L.
dc.contributor.author	Yeo, Jia Hao
dc.contributor.author	Fraser, Stuart T.
dc.contributor.author	New, Elizabeth J.
dc.contributor.author	Bernardes, Goncalo J. L.
dc.contributor.author	Jolliffe, Katrina A.
dc.date.accessioned	2019-12-18
dc.date.available	2019-12-18
dc.date.issued	2018-12-13
dc.identifier.citation	Zwicker, V. E., Oliveira, B. L., Yeo, J. H., Fraser, S. T., Bernardes, G. J. L., New, E. J., & Jolliffe, K. A. (2018). A Fluorogenic Probe for Cell Surface Phosphatidylserine Using an Intramolecular Indicator Displacement Sensing Mechanism. Angewandte Chemie International Edition, 58(10), 3087–3091. https://doi.org/10.1002/anie.201812489	en
dc.identifier.uri	https://hdl.handle.net/2123/21543
dc.description	The detection of externalized phosphatidylserine (PS) on the cell surface is commonly used to distinguish between living, apoptotic, and necrotic cells. The tools of choice for many researchers to study apoptosis are annexin Vfluorophore conjugates. However, the use of this 35 kDa protein is associated with several drawbacks, including temperature sensitivity, Ca2+ dependence, and slow binding kinetics. Herein, a fluorogenic probe for cell surface PS, P-IID, is described, which operates by an intramolecular indicator displacement (IID) mechanism. An intramolecularly bound coumarin indicator is released in the presence of cell surface PS, leading to a fluorescence “turn-on” response. P-IID demonstrates superior performance when compared to annexin V, for both fluorescence imaging and flow cytometry. This allows P-IID to be used in time-lapse imaging of apoptosis using confocal laser scanning microscopy and demonstrates the utility of the IID mechanism in live cells.	en
dc.language.iso	en_AU	en
dc.publisher	Wiley	en
dc.relation	ARC DP140100227, DP180101353	en
dc.rights	Other	en
dc.subject	apoptosis	en
dc.subject	fluorescent probes	en
dc.subject	imaging agents	en
dc.subject	phopshatidylserine	en
dc.title	A fluorogenic probe for cell surface phosphatidylserine using an intramolecular indicator displacement sensing mechanism	en
dc.type	Article	en
dc.subject.asrc	FoR::030302 - Nanochemistry and Supramolecular Chemistry	en
dc.subject.asrc	FoR::030503 - Organic Chemical Synthesis	en
dc.identifier.doi	DOI: 10.1002/anie.201812489
dc.type.pubtype	Author accepted manuscript	en
usyd.faculty	Faculty of Science, School of Chemistry