|Title:||Fluorinated tripodal receptors for potentiometric chloride detection in biological fluids|
Jowett, Laura A.
Howe, Ethan N.W.
Gale, Philip A.
Crespo, Gastón A.
|Citation:||Nadezda Pankratova, Maria Cuartero, Laura A. Jowett, Ethan N.W. Howe, Philip A. Gale, Eric Bakker, Gastón A. Crespo, Fluorinated tripodal receptors for potentiometric chloride detection in biological fluids,Biosensors and Bioelectronics, Volume 99,2018,Pages 70-76,ISSN 0956-5663,https://doi.org/10.1016/j.bios.2017.07.001.|
|Abstract:||Fluorinated tripodal compounds were recently reported to be efficient transmembrane transporters for a series of inorganic anions. In particular, this class of receptors has been shown to be suitable for the effective complexation of chloride, nitrate, bicarbonate and sulfate anions via hydrogen bonding. The potentiometric properties of urea and thiourea-based fluorinated tripodal receptors are explored here for the first time, in light of the need for reliable sensors for chloride monitoring in undiluted biological fluids. The ion selective electrode (ISE) membranes with tren-based tris-urea bis(CF3) tripodal compound (ionophore I) were found to exhibit the best selectivity for chloride over major lipophilic anions such as salicylate (log K = + 1.0 Cl Sal pot −/ − ) and thiocyanate (log K = + 0.1 Cl SCN pot −/ − ). Ionophore I-based ISEs were successfully applied for chloride determination in undiluted human serum as well as artificial serum sample, the slope of the linear calibration at the relevant background of interfering ions being close to Nernstian (49.8 ± 1.7 mV). The results of potentiometric measurements were confirmed by argentometric titration. Moreover, the ionophore I-based ISE membrane was shown to exhibit a very good long-term stability of potentiometric performance over the period of 10 weeks. Nuclear magnetic resonance (NMR) titrations, potentiometric sandwich membrane experiments and density functional theory (DFT) computational studies were performed to determine the binding constants and suggest 1:1 complexation stoichiometry for the ionophore I with chloride as well as salicylate.|
|Rights and Permissions:||© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0|
|Type of Work:||Article|
|Type of Publication:||Post-print|
|Appears in Collections:||Research Papers and Publications. Chemistry|
|Ionophore_paper_v8.pdf||491.08 kB||Adobe PDF|
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