Research Publications and Outputs: Recent submissions
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Fluorinated Analogues of Desferrioxamine B from Precursor-Directed Biosynthesis Provide New Insight into the Capacity of DesBCD
Published 2018The siderophore desferrioxamine B (DFOB, 1) native to Streptomyces pilosus is biosynthesized by the DesABCD enzyme cluster. DesA-mediated decarboxylation of l-lysine gives 1,5-diaminopentane (DP) for processing by DesBCD. ...Open AccessArticle -
Siderophores and iron transport
Published 2023Siderophores are low-molecular-weight organic compounds produced by bacteria and fungi for Fe(III) supply. Bacteria and fungi biosynthesize siderophores in response to low iron, which are released into the local environment ...Book chapter -
Engineering a cleavable disulfide bond into a natural product siderophore using precursor-directed biosynthesis
Published 2018An analogue of the bacterial siderophore desferrioxamine B (DFOB) containing a disulfide motif in the backbone was produced from Streptomyces pilosus cultures supplemented with cystamine. Cystamine competed against native ...Open AccessPre-print -
Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry
Published 2022Converting discrete microbial metabolites into chemical probes for chemical biology and medicinal chemistry studies is typically preceded by lengthy purification and chemical derivatization processes. Standard practice ...Book chapter -
Azido-Desferrioxamine Siderophores as Functional Click-Chemistry Probes Generated in Culture upon Adding a Diazo-Transfer Reagent
Published 2020This work aimed to undertake the in situ conversion of the terminal amine groups of bacterial desferrioxamine (DFO) siderophores, including desferrioxamine B (DFOB), to azide groups to enable downstream click chemistry. ...Open AccessPre-print