• The voltage-sensitive dye RH421 detects a Na+,K+-ATPase conformational change at the membrane interface 

      Garcia, Alvaro; Promod, Pratap R.; Lupfert, Christian; Cornelius, Flemming; Jacquemin, Denis; Lev, Bogdan; Allen, Toby W.; Clarke, Ronald J.
      Published 2017-01-20
      RH421 is a voltage-sensitive fluorescent styrylpyridinium dye which has often been used to probe the kinetics of Na+,K+-ATPase partial reactions. The origin of the dye’s response has up to now been unclear. Here we show ...
      Open Access
      Article
    • Cholesterol depletion inhibits Na+,K+-ATPase activity in a near-native membrane environment 

      Garcia, Alvaro; Lev, Bogdan; Hossain, Khondker R.; Gorman, Amy; Diaz, Dil; Pham, T. H. Nguyen; Cornelius, Flemming; Allen, Toby W.; Clarke, Ronald J.
      Published 2019-02-15
      Cholesterol’s effects on Na+,K+-ATPase reconstituted in phospholipid vesicles have been extensively studied. However, previous studies have reported both cholesterol-mediated stimulation and inhibition of Na+,K+-ATPase ...
      Open Access
      Article
    • Stimulation of Na+,K+-ATPase activity as a possible driving foce in cholesterol evolution 

      Lambropoulos, Nicholas; Garcia, Alvaro; Clarke, Ronald J.
      Published 2015-12-29
      Cholesterol is exclusively produced by animals and is present in the plasma membrane of all animal cells. In contrast, the membranes of fungi and plants contain other sterols. To explain the exclusive preference of animal ...
      Open Access
      Article
    • Interaction of N-terminal peptide analogues of the Na+,K+-ATPase with membranes 

      Nguyen, Khoa; Garcia, Alvaro; Sani, Marc-Antoine; Diaz, Dil; Dubey, Vikas; Clayton, Daniel; Dal Poggetto, Giovanni; Cornelius, Flemming; Payne, Richard J.; Separovic, Frances; Khandelia, Himanshu; Clarke, Ronald J.
      Published 2018-03-06
      The Na+,K+-ATPase, which is present in the plasma membrane of all animal cells, plays a crucial role in maintaining the Na+ and K+ electrochemical potential gradients across the membrane. Recent studies have suggested that ...
      Open Access
      Article
    • Redox-dependent regulation of the Na+-K+ pump: New twists to an old target for treatment of heart failure 

      Liu, Chia-Chi; Fry, Natasha A. S.; Hamilton, Elisha J.; Chia, Karin K. M.; Garcia, Alvaro; Galougahi, Keyvan K.; Figtree, Gemma A.; Clarke, Ronald J.; Bundgaard, Henning; Rasmussen, Helge H.
      Published 2013-05-30
      By the time it was appreciated that the positive inotropic effect of cardiac glycosides is due to inhibition of the membrane Na+-K+ pump, glycosides had been used for treatment of heart failure on an empiric basis for ~200 ...
      Open Access
      Article