• Evidence for ATP interaction with phosphatidylcholine bilayers 

      Garcia, Alvaro; Pochinda, Simon; Elgaard-Jorgensen, Paninnguaq N.; Khandelia, Himanshu; Clarke, Ronald J.
      Published 2019-07-10
      ATP is a fundamental intracellular molecule and is thought to diffuse freely throughout the cytosol. Evidence obtained from nucleoside-sensing sarcolemmal ion channels and red blood cells, however, suggest that ATP is ...
      Open Access
      Article
    • Identification of electric-field-dependent steps in the Na+,K+-pump cycle 

      Mares, Laura J.; Garcia, Alvaro; Rasmussen, Helge H.; Cornelius, Flemming; Mahmmoud, Yasser A.; Berlin, Joshua R.; Lev, Bogdan; Allen, Toby W.; Clarke, Ronald J.
      Published 2014-09-01
      The charge transporting activity of the Na+,K+-ATPase depends on its surrounding electric field. To isolate which steps of the enzyme’s reaction cycle involve charge movement we have investigated the response of the ...
      Open Access
      Article
    • Quantitative calculation of the role of the Na+,K+-ATPase in thermogenesis 

      Clarke, Ronald J.; Catauro, Michelina; Rasmussen, Helge H.; Apell, Hans-Jurgen
      Published 2013-07-11
      The Na+,K+-ATPase is accepted as an important source of heat generation (thermogenesis) in animals. Based on information gained on the kinetics of the enzyme’s partial reactions we consider via computer simulation whether ...
      Open Access
      Article
    • Glutathionylation-dependence of N+-K+-pump currents can mimic reduced subsarcolemmal Na+ diffusion 

      Garcia, Alvaro; Liu, Chia-Chi; Cornelius, Flemming; Clarke, Ronald J.; Rasmussen, Helge H.
      Published 2016-03-01
      The existence of a subsarcolemmal space with restricted diffusion for Na+ in cardiac myocytes has been inferred from a transient peak electrogenic Na+-K+ pump current beyond steady state on re-exposure of myocytes to K+ ...
      Open Access
      Article
    • Extracellular allosteric Na+ binding to the Na+,K+-ATPase in cardiac myocytes 

      Garcia, Alvaro; Fry, Natasha A. S.; Karimi, Keyvan; Liu, Chia-chi; Apell, Hans-Jurgen; Rasmussen, Helge H.; Clarke, Ronald J.
      Published 2013-12-01
      Whole cell patch clamp measurements of the current, Ip, produced by the Na+,K+-ATPase across the plasma membrane of rabbit cardiac myocytes show an increase in Ip over the extracellular Na+ concentration range 0 – 50 mM. ...
      Open Access
      Article