Tagging of Pseudomonas putida using cumate-inducible GFP

Abstract

Green fluorescent protein (GFP) has been used as a reporter protein in many experiments. Previous work has demonstrated that the cumate-inducible gene expression system from Pseudomonas putida F1 works in both Gram negative and Gram positive bacteria (Kaczmarczyk et al (2013), Seo and Schmidt-Dannert (2019)). Here, a plasmid providing cumate-controlled GFP expression was introduced into Pseudomonas putida strain KT2440 (a model rhizosphere bacterium) and the system tested for monitoring plant root colonization.