Supramolecular methods: the chloride/nitrate transmembrane exchange assay

Abstract

The chloride/nitrate anion exchange assay is one of the most common methods of measuring chloride transport across lipid bilayers. In this methods paper, we provide a step-by-step procedure for using this assay based on ion selective electrodes and highlight the advantages and limitations of this approach in assessing the transmembrane chloride transport capability of anionophores.