A sensitive and specific β-alanyl aminopeptidase-activated fluorogenic probe for the detection of Pseudomonas aeruginosa

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Open Access

Field	Value	Language
dc.contributor.author	Váradi, Linda
dc.contributor.author	Hibbs, David E.
dc.contributor.author	Orenga, Sylvain
dc.contributor.author	Babolat, Michèle
dc.contributor.author	Perry, John D.
dc.contributor.author	Groundwater, Paul W.
dc.date.accessioned	2016-04-13
dc.date.available	2016-04-13
dc.date.issued	2016-04-13
dc.identifier.uri	http://hdl.handle.net/2123/14693
dc.description.abstract	We report the synthesis of the sensitive and specific fluorogenic self-immolative substrate 8b, which is hydrolzyed by β-alanyl aminopeptidase (BAP), resulting in a 1,6-elimination and the release of the highly fluorescent hydroxycoumarin 6b. This fluorophore 6b is retained within bacterial colonies, so has potential for the detection of P. aeruginosa (a BAP producer); it also has potential in liquid media due to the rapid and strong signal release from the substrate 8b, and lack of self-quenching or photobleaching.	en
dc.description.sponsorship	NHMRC	en
dc.language.iso	en_AU	en
dc.relation	NHMRC APP1043746	en
dc.rights	Other
dc.subject	P. aeruginosa	en
dc.subject	fluorescence	en
dc.subject	detection	en
dc.subject	β-alanyl aminopeptidase	en
dc.title	A sensitive and specific β-alanyl aminopeptidase-activated fluorogenic probe for the detection of Pseudomonas aeruginosa	en
dc.type	Article	en
dc.subject.asrc	FoR::030401 - Biologically Active Molecules	en
dc.type.pubtype	Pre-print	en
usyd.faculty	Faculty of Medicine and Health, Sydney Pharmacy School	en