|Title:||A sensitive and specific β-alanyl aminopeptidase-activated fluorogenic probe for the detection of Pseudomonas aeruginosa|
Hibbs, David E.
Perry, John D.
Groundwater, Paul W.
|Abstract:||We report the synthesis of the sensitive and specific fluorogenic self-immolative substrate 8b, which is hydrolzyed by β-alanyl aminopeptidase (BAP), resulting in a 1,6-elimination and the release of the highly fluorescent hydroxycoumarin 6b. This fluorophore 6b is retained within bacterial colonies, so has potential for the detection of P. aeruginosa (a BAP producer); it also has potential in liquid media due to the rapid and strong signal release from the substrate 8b, and lack of self-quenching or photobleaching.|
|Type of Work:||Article|
|Type of Publication:||Pre-print|
|Appears in Collections:||Research Papers and Publications. Pharmacy|
|LVetal_1342016preprint.pdf||2.39 MB||Adobe PDF|
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