• Production of inhalation phage powders using spray freeze drying and spray drying techniques for treatment of respiratory infections 

      Leung, Sharon SY; Parumasivam, Thaigarajan; Gao, Fiona G; Carrigy, Nicholas B; Vehring, Reinhard; Finlay, Warren H; Morales, Sandra; Britton, Warwick J; Kutter, Elizabeth; Chan, Hak-Kim
      Published 2016-02-29
      Purpose The potential of aerosol phage therapy for treating lung infections has been demonstrated in animal models and clinical studies. This work compared the performance of two dry powder formation techniques, spray ...
      Open Access
      Article
    • Jet nebulization of bacteriophages with different tail morphologies – structural effects 

      Leung, Sharon SY; Carrigy, Nicholas B; Vehring, Reinhard; Finlay, Warren H; Morales, Sandra; Carter, Elizabeth A; Britton, Warwick J; Kutter, Elizabeth; Chan, Hak-Kim
      Published 2019-01-10
      It was previously demonstrated that the loss of infectivity of a myovirus PEV44 after jet nebulization was closely related to a change in bacteriophage (phage) structure. In this follow-up study, we further examined the ...
      Open Access
      Article
    • Effects of storage conditions on the stability of spray dried, inhalable bacteriophage powders 

      Leung, Sharon SY; Parumasivam, Thaigarajan; Gao, Fiona G; Carrigy, Nicholas B; Vehring, Reinhard; Finlay, Warren H; Morales, Sandra; Britton, Warwick J; Kutter, Elizabeth; Chan, Hak-Kim
      Published 2017-04-15
      This study aimed to develop inhalable powders containing phages active against antibiotic-resistant Pseudomonas aeruginosa for pulmonary delivery. A Pseudomonas phage, PEV2, was spray dried into powder matrices comprising ...
      Open Access
      Article
    • Microfluidic-assisted bacteriophage encapsulation into liposomes 

      Leung, Sharon SY; Morales, Sandra; Britton, Warwick; Kutter, Elizabeth; Chan, Hak-Kim
      Published 2018-07-10
      Microfluidics has recently emerged as a new method of manufacturing liposomes, which allows reproducible mixing in miliseconds on the nanoliter scale. Here we investigated the feasibility of a microfluidic flow focusing ...
      Open Access
      Article
    • Nebulization effects on structural stability of bacteriophage PEV 44 

      Astudillo, Ariel; Leung, Sharon Shui Yee; Kutter, Elizabeth; Morales, Sandra; Chan, Hak-Kim
      Published 2018-01-17
      Reduced infectivity of phage due to the nebulization process has been reported previously, but no visual evidence on structural change upon nebulization has been established, or whether these structural changes can be ...
      Open Access
      Article