Please use this identifier to cite or link to this item:
|Title:||The Z stack of a late brachiolaria larva of the asterinid sea star Parvulastra exigua recorded by confocal laser scanning microscopy|
|Authors:||Morris, Valerie B|
School of Biological Sciences
|Abstract:||The Z stack in this archive is representative of a late brachiolaria larva of P. exigua recorded in the Olympus FluoView 1000 laser scanning system. It is from a study of the morphology of the coeloms and their interconnexions in a pre-metamorphic brachiolaria larva. The data of the Z stack show the coelom on the left and right sides of the larva linked anteriorly, over the head of the archenteron, by a common anterior coelom. The asymmetry of the larger left and the smaller right larval coeloms translates into the oral and aboral coeloms of the juvenile sea star. The morphology of the coeloms in a late brachiolaria larva of an asteroid has application in the search for morphological homology between the echinoderm classes and the deuterostome phyla. ***** Late brachiolaria larvae of P. exigua were fixed seven days after fertilization in 2.5% (v/v) glutaraldehyde in filtered sea water for 1-2 hours, dehydrated in an ethanol series to 100% ethanol, then cleared in 2:1 (v/v) benzyl benzoate/benzyl alcohol. The larvae, made autofluorescent by glutaraldehyde fixation, were mounted in the clearant in a chamber between glass coverslips that sealed a hole cut through a conventional microscope slide. The larvae were viewed in the Olympus FluoView 1000 laser scanning system (version 184.108.40.206) attached to an Olympus IX81 inverted microscope. Each larva was excited with a 633 nm helium-neon laser with the emission collected from 645-745 nm. A stack of images was collected along the Z axis (the Z stack) with a pixel dimension of 1.24 x 1.24 µm and a slice thickness of 1.10 µm averaged over two frames with a 20x UplanApo objective lens NA 0.7 in a 512 x 512 pixel array, 12 bits/pixel.|
|Description:||The Z stack is a series of images scanned in the XY plane at successive levels through the depth of the body of the larva. The orientation of the larva is such that view is of the larval ventral surface and larval anterior is on the left in each image. Since the images were reflected by the IX81 inverted microscope, the images should be reflected to obtain the correct left-right view. The images are tif images saved in sequence into one folder. Each tif image can be viewed individually or the series of tif images contained in the folder can be analysed by image analysis software such as ImageJ available from the National Institutes of Health site (http://rsbweb.nih.gov/ij/). Scale bar 100 µm.|
|Department/Unit/Centre:||School of Biological Sciences|
|Type of Work:||Dataset|
|Appears in Collections:||Research Papers and Publications. Biological Sciences|
This work is protected by Copyright. All rights reserved. Access to this work is provided for the purposes of personal research and study. Except where permitted under the Copyright Act 1968, this work must not be copied or communicated to others without the express permission of the copyright owner. Use the persistent URI in this record to enable others to access this work.
|ex051008_1.zip||Zstack folder (compressed)||14.81 MB||Zip||View/Open|
|ex051008_1.avi||Video of Zstack images||98.31 MB||AVI Video||View/Open|
|ex051008_1avi.zip||Zipped (compressed) avi video||13.11 MB||Zip||View/Open|
|ex051008_1sb_c001z001.tif||First image in the Zstack with a scale bar||795.86 kB||TIFF|
Items in Sydney eScholarship Repository are protected by copyright, with all rights reserved, unless otherwise indicated.