Warming but not food limitation alters metabolism during larval development in crown-of-thorns sea stars (Acanthaster sp.)
| Field | Value | Language |
| dc.contributor.author | Hill, Ronan Thomas | |
| dc.contributor.author | Byrne, Maria | |
| dc.contributor.author | Pettersen, Amanda | |
| dc.coverage.temporal | 2025 | en |
| dc.date.accessioned | 2026-04-22T06:14:27Z | |
| dc.date.available | 2026-04-22T06:14:27Z | |
| dc.date.issued | 2026-04-22 | |
| dc.identifier.uri | https://hdl.handle.net/2123/35124 | |
| dc.description.abstract | Respiration data of Acanthaster sp. reared as both fed and unfed larvae at four temperature treatments (control: 26°C; warm: 28°C, 30°C, 32°C) based on current and projected OW and measured metabolic rate (oxygen consumption, V O2) across development to the late larval stage. Dataset includes size (larval area, length and width), developmental stage and plate reader details. | en |
| dc.language.iso | en | en |
| dc.rights | Creative Commons Attribution 4.0 | en |
| dc.subject | COTS | en |
| dc.subject | echinoderm | en |
| dc.subject | larvae | en |
| dc.subject | metabolism | en |
| dc.subject | respiration | en |
| dc.subject | climate change | en |
| dc.subject | metabolic suppression | en |
| dc.title | Warming but not food limitation alters metabolism during larval development in crown-of-thorns sea stars (Acanthaster sp.) | en |
| dc.type | Dataset | en |
| dc.subject.asrc | ANZSRC FoR code::31 BIOLOGICAL SCIENCES | en |
| dc.identifier.doi | 10.25910/1ny6-s981 | |
| dc.description.method | Metabolic rate of individual gastrulae (~ 24 hours post fertilisation – hpf), early bipinnaria (just prior to feeding, ~ 3 dpf), feeding stage bipinnaria (7-14 dpf) and brachiolaria (21 dpf) were measured. The metabolic rates of fed and unfed early and mid bipinnaria from the at 26˚C, 28˚C, 30˚C and 32˚C treatments were measured on day 7 and 14. By the late bipinnaria (14 days) stage only cultures reared at 26˚C, 28˚C, and 30˚C (fed and unfed) could be tested because the 32˚C cultures had 100% mortality. In addition, the unfed cultures died before reaching brachiolaria in all temperature treatments. For the brachiolaria stage (21 dpf) only larvae from fed cultures reared at 26˚C and 28˚C were available for respirometry. Successful development to the brachiolaria was indicated by the formation of the attachment complex which is essential for larval settlement. Metabolic rates were measured as the rate of oxygen consumption (VO2) using closed respirometry. Individual larvae were placed into separate 80-uL wells within 24-well glass microplates (Loligo Systems ApS, Viborg, Denmark) fitted with a 3mm oxygen sensor spot. Fed larvae were hand-picked and placed in clean FSW and starved for 24 hours prior to measurement to ensure they were in a post food-absorptive state to reduce noise in oxygen consumption due to digestion. Examination of the larvae indicated that their stomachs were empty. The 80-uL wells were filled with pasteurised FSW (microwaved for 8 minutes 24 hours prior), sealed and checked to ensure that no air bubbles were present. The microplates were secured onto SensorDish using grip plastic and weight, then placed into a 1L water bath with recirculating water connected to a 30 L header tank at the measurement temperature (26˚C, 28˚C, 30˚C, 32˚C). Percentage oxygen saturation was measured at 1-minute intervals using PreSens Sensor Dish reader (SDR) software (v4) for approximately 3.5 hours or until there was a 20% decrease in oxygen saturation. Empty wells (n=6) without a specimen were used as controls to check for background changes in oxygen levels. Since larvae were mobile during measurements, it is unlikely that localised oxygen depletion occurred in the wells. To determine the effect of body size on metabolic rates, all embryos and larvae were photographed prior to being placed in the respirometry wells using an Olympus compound microscope with an attached DP23 digital microscope camera and Olympus CellSens software. The surface area of the embryos and larvae was measured by tracing to the nearest µm2 using a 1mm scale bar and ImageJ software (ImageJ version 1.54K browser edition). The V ̇O2 for individual gastrulae and larvae were calculated by extracting the slope for all wells selecting for the most linear sections of each slope utilising RespR package in R, with the initial 30 min of measurements removed to allow for accurate depiction of data (Harianto et al., 2019). The average blank slope was calculated based on the control wells and then subtracted from the experimental wells. VO2 (µlO2min-1) was calculated per equation [1], [1] VO2 = -1 (ma - mb)/100 x V x bO2 Where ma is the rate of change of O2 saturation for experimental wells (% per hour), mb is the rate of change of O2 saturation for control wells (% per hour), bO2 is the oxygen capacitance of sea water at the measured temperature (Cameron, 1986 - see Appendix table 2.1), and V is the water volume in the well. | en |
| usyd.faculty | SeS faculties schools::Faculty of Science::School of Life and Environmental Sciences | en |
| workflow.metadata.only | No | en |
Associated file/s
Associated collections