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dc.contributor.authorPeel, E
dc.contributor.authorFrankenberg, S
dc.contributor.authorHogg, CJ
dc.contributor.authorPask, A
dc.contributor.authorBelov, K
dc.date.accessioned2023-06-21T05:49:26Z
dc.date.available2023-06-21T05:49:26Z
dc.date.issued2021en_AU
dc.identifier.issn1432-1211
dc.identifier.urihttps://hdl.handle.net/2123/31377
dc.description.abstractAdvances in genome sequencing technology have enabled genomes of extinct species to be sequenced. However, given the fragmented nature of these genome assemblies, it is not clear whether it is possible to comprehensively annotate highly variable and repetitive genes such as those involved in immunity. As such, immune genes have only been investigated in a handful of extinct genomes, mainly in human lineages. In 2018 the genome of the thylacine (Thylacinus cynocephalus), a carnivorous marsupial from Tasmania that went extinct in 1936, was sequenced. Here we attempt to characterise the immune repertoire of the thylacine and determine similarity to its closest relative with a genome available, the Tasmanian devil (Sarcophilus harrisii), as well as other marsupials. Members from all major immune gene families were identified. However, variable regions could not be characterised, and complex families such as the major histocompatibility complex (MHC) were highly fragmented and located across multiple small scaffolds. As such, at a gene level we were unable to reconstruct full-length coding sequences for the majority of thylacine immune genes. Despite this, we identified genes encoding functionally important receptors and immune effector molecules, which suggests the functional capacity of the thylacine immune system was similar to other mammals. However, the high number of partial immune gene sequences identified limit our ability to reconstruct an accurate picture of the thylacine immune repertoire.en_AU
dc.language.isoenen_AU
dc.publisherSpringer Natureen_AU
dc.relation.ispartofImmunogeneticsen_AU
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0en_AU
dc.subjectMarsupialen_AU
dc.subjectimmunogeneticsen_AU
dc.subjectMHCen_AU
dc.subjectthylacineen_AU
dc.subjectextincten_AU
dc.titleAnnotation of immune genes in the extinct thylacine (Thylacinus cynocephalus)en_AU
dc.typeArticleen_AU
dc.identifier.doi10.1007/s00251-020-01197-z
dc.type.pubtypeAuthor accepted manuscripten_AU
dc.relation.arcDP180102465
usyd.facultySeS faculties schools::Faculty of Science::School of Life and Environmental Sciencesen_AU
usyd.citation.volume73en_AU
usyd.citation.spage263en_AU
usyd.citation.epage275en_AU
workflow.metadata.onlyNoen_AU


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