Patients with treated indolent lymphomas immunized with BNT162b2 have reduced anti‐spike neutralizing IgG to SARS‐CoV‐2 variants, but preserved antigen‐specific T cell responses
Type
ArticleAuthor/s
Beaton, BrendanSasson, Sarah C.
Rankin, Katherine
Raedemaeker, Juliette
Wong, Alexander
Hastak, Priyanka
Phetsouphanh, Chansavath
Warden, Andrew
Klemm, Vera
Munier, C. Mee Ling
Hoppe, Alexandra Carey
Tea, Fiona
Pillay, Aleha
Stella, Alberto Ospina
Aggarwal, Anupriya
Lavee, Orly
Caterson, Ian D.
Turville, Stuart
Kelleher, Anthony D.
Brilot, Fabienne
Trotman, Judith
Abstract
Patients with indolent lymphoma undertaking recurrent or continuous B cell suppression are at risk of severe COVID-19. Patients and healthy controls (HC; N=13) received 2 doses of BNT162b2: Follicular Lymphoma (FL; N=35) who were treatment naïve (TN; N=11) or received immunochemotherapy ...
See morePatients with indolent lymphoma undertaking recurrent or continuous B cell suppression are at risk of severe COVID-19. Patients and healthy controls (HC; N=13) received 2 doses of BNT162b2: Follicular Lymphoma (FL; N=35) who were treatment naïve (TN; N=11) or received immunochemotherapy (ICT; N=23) and Waldenström's Macroglobulinemia (WM; N=37) including TN (N=9), ICT (N=14), or treated with Bruton's Tyrosine Kinase inhibitors (BTKi; N=12). Anti-spike IgG was determined by a high-sensitivity flow-cytometric assay, in addition to live-virus neutralization. Antigen-specific T cells were identified by co-expression of CD69/CD137 and CD25/CD134 on T cells. A subgroup (N=29) were assessed for third mRNA vaccine response, including omicron neutralization. One month after second BNT162b2, median anti-spike IgG mean fluorescence intensity (MFI) in FL ICT patients (9977) was 25-fold lower than TN (245898) and HC (228255, p=0.0002 for both). Anti-spike IgG correlated with lymphocyte count (r=0.63; p=0.002), and time from treatment, (r=0.56; p=0.007) on univariate analysis, but only with lymphocyte count on multivariate analysis (p=0.03). In the WM cohort, median anti-spike IgG MFI in BTKi patients (39039) was reduced compared to TN (220645, p=0.0008) and HC (p<0.0001). Anti-spike IgG correlated with neutralization of the delta variant (r=0.62, p<0.0001). Median neutralization titer for WM BTKi (0) was lower than HC (40, p<0.0001) for early-clade and delta. All cohorts had functional T cell responses. Median anti-spike IgG decreased 4-fold from second to third dose (p=0.004). Only 5/29 poor initial responders assessed after third vaccination demonstrated seroconversion and improvement in neutralization activity, including to the omicron variant. This article is protected by copyright. All rights reserved.
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See morePatients with indolent lymphoma undertaking recurrent or continuous B cell suppression are at risk of severe COVID-19. Patients and healthy controls (HC; N=13) received 2 doses of BNT162b2: Follicular Lymphoma (FL; N=35) who were treatment naïve (TN; N=11) or received immunochemotherapy (ICT; N=23) and Waldenström's Macroglobulinemia (WM; N=37) including TN (N=9), ICT (N=14), or treated with Bruton's Tyrosine Kinase inhibitors (BTKi; N=12). Anti-spike IgG was determined by a high-sensitivity flow-cytometric assay, in addition to live-virus neutralization. Antigen-specific T cells were identified by co-expression of CD69/CD137 and CD25/CD134 on T cells. A subgroup (N=29) were assessed for third mRNA vaccine response, including omicron neutralization. One month after second BNT162b2, median anti-spike IgG mean fluorescence intensity (MFI) in FL ICT patients (9977) was 25-fold lower than TN (245898) and HC (228255, p=0.0002 for both). Anti-spike IgG correlated with lymphocyte count (r=0.63; p=0.002), and time from treatment, (r=0.56; p=0.007) on univariate analysis, but only with lymphocyte count on multivariate analysis (p=0.03). In the WM cohort, median anti-spike IgG MFI in BTKi patients (39039) was reduced compared to TN (220645, p=0.0008) and HC (p<0.0001). Anti-spike IgG correlated with neutralization of the delta variant (r=0.62, p<0.0001). Median neutralization titer for WM BTKi (0) was lower than HC (40, p<0.0001) for early-clade and delta. All cohorts had functional T cell responses. Median anti-spike IgG decreased 4-fold from second to third dose (p=0.004). Only 5/29 poor initial responders assessed after third vaccination demonstrated seroconversion and improvement in neutralization activity, including to the omicron variant. This article is protected by copyright. All rights reserved.
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Date
2022Share