An evaluation of 4 commercial assays for the detection of SARS-CoV-2 antibodies in a predominantly mildly symptomatic low prevalence Australian population
Field | Value | Language |
dc.contributor.author | Wehrhahn, Michael C | en_AU |
dc.contributor.author | Brown, Suzanne J | en_AU |
dc.contributor.author | Newcombe, James P | en_AU |
dc.contributor.author | Chong, Smathi | en_AU |
dc.contributor.author | Evans, Jenny | en_AU |
dc.contributor.author | Figtree, Melanie | en_AU |
dc.contributor.author | Hainke, Laurence | en_AU |
dc.contributor.author | Hueston, Linda | en_AU |
dc.contributor.author | Khan, Sadid | en_AU |
dc.contributor.author | Marland, Elizabeth | en_AU |
dc.contributor.author | O'Sullivan, Matthew V N | en_AU |
dc.contributor.author | Powell, Helen | en_AU |
dc.contributor.author | Roy, Jhumur | en_AU |
dc.contributor.author | Waring, Lynette | en_AU |
dc.contributor.author | Yu, Megan | en_AU |
dc.contributor.author | Robson, Jennifer | en_AU |
dc.date.accessioned | 2021-06-02T04:54:55Z | |
dc.date.available | 2021-06-02T04:54:55Z | |
dc.date.issued | 2021 | |
dc.identifier.uri | https://hdl.handle.net/2123/25195 | |
dc.description.abstract | A total of 1080 individual patient samples (158 positive serology samples from confirmed, predominantly mildly symptomatic COVID-19 patients and 922 serology negative including 496 collected pre-COVID) from four states in Australia were analysed on four commercial SARS-CoV-2 serological assays targeting antibodies to different antigens (Roche Elecsys and Abbott Architect: nucleocapsid; Diasorin Liaison and Euroimmun: spike). A subset was compared to immunofluorescent antibody (IFA) and micro-neutralisation. Sensitivity and specificity of the Roche (n = 1033), Abbott (n = 806), Diasorin (n = 1034) and Euroimmun (n = 175) were 93.7 %/99.5 %, 90.2 %/99.4 %, 88.6 %/98.6 % and 91.3 %/98.8 %, respectively. ROC analysis with specificity held at 99 % increased the sensitivity for the Roche and Abbott assays from 93.7% to 98.7% (cut-off 0.21) and 90.2 % to 94.0 % (cut-off 0.91), respectively. Overall seropositivity of samples increased from a maximum of 23 % for samples 0-7 days-post-onset of symptoms (dpos), to 61 % from samples 8-14dpos and 93 % from those >14dpos. IFA and microneutralisation values correlated best with assays targeting antibodies to spike protein with values >80 AU/mL on the Diasorin assay associated with neutralising antibody. Detectable antibody was present in 22/23 (96 %), 20/23 (87 %), 15/23 (65 %) and 9/22 (41 %) patients with samples >180dpos on the Roche, Diasorin, Abbott and microneutralisation assays respectively. Given the low prevalence in this community, two-step algorithms on initial positive results saw an increase in the positive predictive value (PPV) of positive samples (39 %-65 % to ≥98 %) for all combinations. Similarly accuracy increased from a range of 98.5 %-99.4 % to ≥99.8 % assuming a 1 % seroprevalence. Negative predictive value (NPV) was high (≥99.8 %) regardless of which assay was used initially. | en_AU |
dc.language.iso | en | en_AU |
dc.subject | COVID-19 | en_AU |
dc.subject | Coronavirus | en_AU |
dc.title | An evaluation of 4 commercial assays for the detection of SARS-CoV-2 antibodies in a predominantly mildly symptomatic low prevalence Australian population | en_AU |
dc.type | Article | en_AU |
dc.identifier.doi | 10.1016/j.jcv.2021.104797 |
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