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dc.contributor.authorDi Teodoro, Giovannien_AU
dc.contributor.authorValleriani, Fabriziaen_AU
dc.contributor.authorPuglia, Ilariaen_AU
dc.contributor.authorMonaco, Federicaen_AU
dc.contributor.authorDi Pancrazio, Chiaraen_AU
dc.contributor.authorLuciani, Mirellaen_AU
dc.contributor.authorKrasteva, Ivankaen_AU
dc.contributor.authorPetrini, Antonioen_AU
dc.contributor.authorMarcacci, Mauriliaen_AU
dc.contributor.authorD’Alterio, Nicolaen_AU
dc.contributor.authorCurini, Valentinaen_AU
dc.contributor.authorIorio, Mariangelaen_AU
dc.contributor.authorMigliorati, Giacomoen_AU
dc.contributor.authorDi Domenico, Marcoen_AU
dc.contributor.authorMorelli, Danielaen_AU
dc.contributor.authorCalistri, Paoloen_AU
dc.contributor.authorSavini, Giovannien_AU
dc.contributor.authorDecaro, Nicolaen_AU
dc.contributor.authorHolmes, Edward C.en_AU
dc.contributor.authorLorusso, Alessioen_AU
dc.date.accessioned2020-12-21
dc.date.available2020-12-21
dc.date.issued2021en_AU
dc.identifier.urihttps://hdl.handle.net/2123/24217
dc.description.abstractThere is strong evidence that severe acute respiratory syndrome 2 virus (SARS-CoV-2), the causative agent of the coronavirus disease 2019 (COVID-19) pandemic, originated from an animal reservoir. However, the exact mechanisms of emergence, the host species involved, and the risk to domestic and agricultural animals are largely unknown. Some domestic animal species, including cats and ferrets, have been demonstrated to be susceptible to SARS-CoV-2 infection, while others, such as pigs and chickens, are not. Importantly, the susceptibility of ruminants to SARS-CoV-2 is unknown, even though they often live in close proximity to humans. We investigated the replication and tissue tropism of two different SARS-CoV-2 isolates in the respiratory tract of three farm animal species - cattle, sheep, and pigs - using respiratory ex vivo organ cultures (EVOCs). We demonstrate that the respiratory tissues of cattle and sheep, but not of pigs, sustain viral replication in vitro of both isolates and that SARS-CoV-2 binds efficiently to ACE2-expressing cells of the respiratory tract of both ruminant species. Intriguingly, SARS-CoV-2 isolates containing an amino acid substitution at site 614 of the spike protein (mutation D614?G) replicated at higher magnitude in ex vivo tissues of both ruminant species, supporting previous results obtained using human cells. These results suggest that additional in vivo experiments involving several ruminant species are warranted to determine the potential role in the epidemiology of this virus.en_AU
dc.language.isoenen_AU
dc.subjectCOVID-19en_AU
dc.subjectCoronavirusen_AU
dc.titleSARS-CoV-2 replicates in respiratory ex vivo organ cultures of domestic ruminant speciesen_AU
dc.typeArticleen_AU
dc.identifier.doi10.1016/j.vetmic.2020.108933
dc.relation.arcFL170100022


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