|Title:||Tropomyosin Tm5NM1 spatially restricts src kinase activity through perturbation of Rab11 vesicle trafficking|
|Authors:||Bach, Cuc T.|
Murray, Rachael Z.
O'Neill, Geraldine M.
Discipline of Pharmacology
|Publisher:||Molecular and Cellular Biology|
|Citation:||Bach CT, Murray RZ, Owen D, Gaus K, O’Neill GM. Tropomyosin Tm5NM1 Spatially Restricts Src Kinase Activity through Perturbation of Rab11 Vesicle Trafficking. Molecular and Cellular Biology. 2014;34(24):4436-4446. doi:10.1128/MCB.00796-14.|
|Abstract:||In order for cells to stop moving, they must synchronously stabilize actin filaments and their associated focal adhesions. How these two structures are coordinated in time and space is not known. We show here that the actin association protein Tm5NM1, which induces stable actin filaments, concurrently suppresses the trafficking of focal-adhesion-regulatory molecules. Using combinations of fluorescent biosensors and fluorescence recovery after photobleaching (FRAP), we demonstrate that Tm5NM1 reduces the level of delivery of Src kinase to focal adhesions, resulting in reduced phosphorylation of adhesion-resident Src substrates. Live imaging of Rab11-positive recycling endosomes that carry Src to focal adhesions reveals disruption of this pathway. We propose that tropomyosin synchronizes adhesion dynamics with the cytoskeleton by regulating actin-dependent trafficking of essential focal-adhesion molecules.|
|Department/Unit/Centre:||Discipline of Pharmacology|
|Type of Work:||Article|
|Type of Publication:||Publisher's version|
|Appears in Collections:||Research Papers and Publications. Discipline of Pharmacology|
|Bach_Mol_Cell_Biol_2014.pdf||2.77 MB||Adobe PDF|