Pre-analytical stability of antibodies to pathogenic antigens
Field | Value | Language |
dc.contributor.author | Hodgkinson VS | en_AU |
dc.contributor.author | Egger S | en_AU |
dc.contributor.author | Betsou F | en_AU |
dc.contributor.author | Waterboer T | en_AU |
dc.contributor.author | Pawlita M | en_AU |
dc.contributor.author | Michel A | en_AU |
dc.contributor.author | Baker MS | en_AU |
dc.contributor.author | Banks E | en_AU |
dc.contributor.author | Sitas F | en_AU |
dc.date.issued | 2017 | |
dc.date.issued | 2017 | en |
dc.identifier.uri | https://hdl.handle.net/2123/30904 | |
dc.description.abstract | Background: Serologic testing for antibodies against epitopes from pathogens is a valuable tool for investigating the relationship between infection and disease. This study comprehensively evaluates the impact of preanalytic variation on antibody seropositivities to a selected set of antigens arising from delays in processing of blood samples, preprocessing storage temperature, and vacutainer type. Methods: We assessed peripheral blood collected from 29 volunteers in four different Vacutainer types [ethylenediaminoetetraacetic acid (EDTA), acid-citrate-dextrose (ACD), lithium heparin (LH), serum separator tubes (SST)], and stored at 4°C or room temperature for 0, 1, 2, 3, 4, 5, and 6 days before processing. Multiplex serology was used to determine antibody reactivity against 35 antigens derived from human papillomaviruses, human polyomaviruses, Epstein–Barr virus, and Helicobacter pylori. Cohen's κ statistic was used to measure agreement on seropositivity status between samples exposed to standard and nonstandard clinical practice conditions. Results: For samples processed without delay, κ was not associated with storage-temperature (P value range 0.23 to 0.95) or vacutainer type (P value range, 0.35–0.89). Kappa did not significantly decline with increasing delays in processing for any vacutainer-type storage temperature combination (P slope range, 0.06–1.00). Conclusions: Antibodies to epitopes from various pathogenic infectious agents can be measured reliably from samples stored in SST, EDTA, ACD, or LH vacutainers at either room temperature or 4°C for up to 6 days before processing. Impact: Serologic testing is robust to several preanalytic options. These findings are particularly important for epidemiologic studies recruiting participants from remote settings where sample exposure to preanalytic conditions can vary considerably. | en_AU |
dc.publisher | Cancer Epidemiology, Biomarkers & Prevention | en_AU |
dc.subject.other | Prevention - Resources and Infrastructure | en_AU |
dc.title | Pre-analytical stability of antibodies to pathogenic antigens | en_AU |
dc.type | Article | en_AU |
dc.identifier.doi | 10.1158/1055-9965.EPI-17-0170 |
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