19F NMR of erythrocytes: 'split peak' phenomenon, membrane potential and membrane transport
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Open Access
Type
ThesisThesis type
Doctor of PhilosophyAuthor/s
Xu, Arron Si LaoAbstract
The general phenomenon of the transmembr ane 19F NMR chemical shift difference that arises with a number of fluorinated solutes when they are added to erythrocyte suspensions, erythrocyte ghosts and lipid vesicles has been explored. Chapter 1 gives a general introductio n to NMR ...
See moreThe general phenomenon of the transmembr ane 19F NMR chemical shift difference that arises with a number of fluorinated solutes when they are added to erythrocyte suspensions, erythrocyte ghosts and lipid vesicles has been explored. Chapter 1 gives a general introductio n to NMR phenomena, erythrocyte membrane electrochem ical potential, and erythrocyt e-membrane transport of anions and monocarboxy lates. A discussion of NMR-based methods for measuring membrane transport in included. General materials and methods that are used throughout the thesis are given in Chapter 2. Chapter 3 introduces the phenomenon of transmembrane chemical-shift difference of 19F and 31P NMR resonances from solutes added to erythrocyte suspensions. In Chapter 4, there is a detailed discussion of the principal physical basis for the 'split peak' phenomenon . This phenomeno n was used to determine the electrochem ical equilibrium distribution of difluoropho sphate, a fluorinated analogue of orthophosph ate, in erythrocyte suspensions. Chapter 5 describes the measurement of erythrocyte membrane potential from estimates of the equilibrium distribution of difluorophosphate. Chapter 6 deals with the study of rapid exchange of difluorophosphate across erythrocyte membranes, by using a novel approach that only measures the magnetisation exchange between the transmembrane solute-populations, and not between the population components of the 31F split doublet. This was achieved by simultaneously perturbing the doublet components of the solute of cis compartment . In Chapter 7, the various pathways involved in the transport of trifluoroacet ate across the human erythrocyte membrane are discussed. Chapter 8 has a description of 9Be and 19P NMR studies of the multiple equilibrium between beryllofluoride complexes in aqueous media, and their uptake by human erythrocytes . A general discussion of the work presented, and the future directions of research, are given in Chapter 9.
See less
See moreThe general phenomenon of the transmembr ane 19F NMR chemical shift difference that arises with a number of fluorinated solutes when they are added to erythrocyte suspensions, erythrocyte ghosts and lipid vesicles has been explored. Chapter 1 gives a general introductio n to NMR phenomena, erythrocyte membrane electrochem ical potential, and erythrocyt e-membrane transport of anions and monocarboxy lates. A discussion of NMR-based methods for measuring membrane transport in included. General materials and methods that are used throughout the thesis are given in Chapter 2. Chapter 3 introduces the phenomenon of transmembrane chemical-shift difference of 19F and 31P NMR resonances from solutes added to erythrocyte suspensions. In Chapter 4, there is a detailed discussion of the principal physical basis for the 'split peak' phenomenon . This phenomeno n was used to determine the electrochem ical equilibrium distribution of difluoropho sphate, a fluorinated analogue of orthophosph ate, in erythrocyte suspensions. Chapter 5 describes the measurement of erythrocyte membrane potential from estimates of the equilibrium distribution of difluorophosphate. Chapter 6 deals with the study of rapid exchange of difluorophosphate across erythrocyte membranes, by using a novel approach that only measures the magnetisation exchange between the transmembrane solute-populations, and not between the population components of the 31F split doublet. This was achieved by simultaneously perturbing the doublet components of the solute of cis compartment . In Chapter 7, the various pathways involved in the transport of trifluoroacet ate across the human erythrocyte membrane are discussed. Chapter 8 has a description of 9Be and 19P NMR studies of the multiple equilibrium between beryllofluoride complexes in aqueous media, and their uptake by human erythrocytes . A general discussion of the work presented, and the future directions of research, are given in Chapter 9.
See less
Date
1992Rights statement
The author retains copyright of this thesis. It may only be used for the purposes of research and study. It must not be used for any other purposes and may not be transmitted or shared with others without prior permission.Department, Discipline or Centre
Department of BiochemistryAwarding institution
The University of SydneyShare