|Title:||The morphological and genetic characterisation of pelvic finless zebrafish|
|Authors:||Don, Emily Kate|
|Keywords:||Pelvic fin, development|
|Publisher:||University of Sydney.|
Anatomy and Histology.
School of Medical Sciences
|Abstract:||This thesis aims to establish the foundations for future developmental studies in the pelvic fins of zebrafish by characterising pelvic fin development and describing a novel pelvic finless zebrafish. The pelvic finless strain of zebrafish represents a naturally occurring phenotype where the adult zebrafish lack pelvic fins. In order to characterise the absence of pelvic fins in these fish, the morphology and gene expression patterns of pelvic fin development were examined and compared to wild-type zebrafish. It was shown that, unlike wild-type fish, pelvic finless zebrafish do not form the apical ectodermal thickening of the pelvic fin bud and fin development is subsequently arrested. Genetic mapping and targeted next generation sequencing revealed three candidate missense mutations in a striking candidate gene, tbx4, that are likely to underlie the loss of pelvic fins in pelvic finless zebrafish. Ongoing transgenic rescue experiments, DNA binding assays and knockdown experiments are hoped to confirm that mutations in tbx4 do in fact underlie the pelvic finless phenotype. This work represents a novel characterisation and finding in the field of pelvic fin development and is hoped to contribute to the genetic understanding of pelvic fin and hindlimb development.|
|Type of Work:||PhD Doctorate|
|Type of Publication:||Doctor of Philosophy Ph.D.|
|Appears in Collections:||Sydney Digital Theses (Open Access)|
|DON Emily - Final thesis.pdf||16.26 MB||Adobe PDF|
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