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|Title: ||Analysis of placental gene expression profile in pregnancies affected by fetal growth restriction and macrosomia|
|Authors: ||Sabri, Amin|
|Issue Date: ||5-Dec-2013|
|Publisher: ||University of Sydney. Central Clinical School|
|Abstract: ||Introduction: Extremes of fetal growth are associated with increased perinatal mortality and morbidity and a higher prevalence of cardiovascular disease, obesity and diabetes in later life. In this study, our aim was to identify changes in placental gene expression with evidence of growth dysfunction and to identify candidate genes that may be used to identify abnormal patterns of growth prior to delivery.
Materials and methods: Placentas were collected from growth restricted (n=4), macrosomic (n=6) and normal term pregnancies (n=5). RNA was extracted prior to microarray analysis using Affymetrix HG-U219 arrays to determine variation in gene expression. Genes of interest confirmed using qRT-PCR.
Results: 338 genes in the growth restricted and 41 genes in the macrosomic group were identified to be significantly dysregulated (>2 fold change; p<0.05). CPXM2 and CLDN1 were up-regulated and TXNDC5 and LRP2 down-regulated in fetal growth restriction. In macrosomia, PHLDB2 and CLDN1 were up- and LEP and GCH1 were down-regulated.
Discussion: Dysfunctional growth is associated with differential placental gene expression and affects genes involved with a whole spectrum of developmental and cellular functions. Better elucidation of these pathways may allow development of biomarkers able to identify growth abnormalities prenatally and allow effective intervention reducing both short and long term morbidity.|
|Type of Work: ||Masters Thesis|
|Type of Publication: ||Master of Philosophy M.Phil|
|Appears in Collections:||Sydney Digital Theses (Open Access)|
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|SABRI Amin - Final thesis.pdf||3.74 MB||Adobe PDF|
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