This thesis entitled “French Horn Players’ Attitudes Regarding the Accurate Application of Anatomical Knowledge in Optimal Playing Philosophies” written in requirement of a Master of Philosophy (Medicine), University of Sydney, was instigated by a desire to overcome my own artistic limitations as a French Horn player and to explore the role accurately applied anatomical knowledge might play in understanding and overcoming these limitations. Through a literature review, concepts around the application of accurate anatomical information from various sources to inform optimal playing philosophies were explored. An in-depth understanding of anatomical structures was acquired and followed by the development of 3D modelled anatomical resources in consultation with Dr Bronwen Ackermann, PhD, MPH, BAppSc(PT) and Dr Mark Halaki, PhD, MSc, Bsc. These were used as a resource to support gathering both qualitative and quantitative data. The attitudes of French Horn players were examined in a mixed method study including surveys and in-depth interviews with professional and highly regarded French Horn players. Data showed that French Horn players have limited knowledge of functional anatomy and that this could potentially contribute to the development of Playing Related Medical Disorders (PRMDs). The study also identified that aspiring horn players would value more knowledge of functional anatomy, demonstrated the important role of medical professionals in mediating this knowledge, and recommends that it would be appropriate that study of functional anatomy be incorporated into tertiary study of the French Horn, however applicability, appropriacy and context are also contributing factors to the usefulness of this data.