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dc.contributor.authorBeekman, Anneke
dc.date.accessioned2019-09-11
dc.date.available2019-09-11
dc.date.issued2019-02-28
dc.identifier.urihttp://hdl.handle.net/2123/21092
dc.descriptionIncludes publicationsen_AU
dc.description.abstractBackground MatriDerm, a collagen-elastin dermal template, is commonly used in full-thickness wound repair, to promote dermal regeneration and improve scar tissue quality. Due to the non-cross-linked status, it is expected to biodegrade relatively quickly. Cross-linking the collagen-elastin template could potentially enhance its performance. Aim The aim of this study was to investigate the effects of cross-linking on MatriDerm stability, cell interaction, biodegradation and wound contraction using established cell culture and murine models. Method MatriDerm was cross-linked with glutaraldehyde vapour and characterized in comparison with noncross- linked MatriDerm. Surface morphology, in-vitro stability and strength were assessed through scanning electron microscopy, measurement of protein loss, and tensile modulus testing, respectively. Cell-scaffold interaction, cell proliferation and migration was examined using cultured human dermal fibroblasts. The scaffold biodegradation and its impact on wound healing and contraction was studied in a murine model. Results Cross-linked MatriDerm displayed a slight but significant reduction in average pore size, a significant reduction of total protein loss and a 3-fold increase in tensile strength compared to the non-crosslinked template. In-vitro studies observed a significant increase of fibroblast proliferation and migration in cross-linked MatriDerm and reduced scaffold contraction compared to non-cross-linked MatriDerm. In the murine model, non-cross-linked MatriDerm was almost completely biodegraded after 14 days but cross-linked MatriDerm remained intact, demonstrating similar host responses. Conclusion Cross-linked MatriDerm’s durability was enhanced, demonstrated by significant increase in biostability and strength both in-vitro and in-vivo. The extended exposure of cross-linked MatriDerm in a wound could have beneficial effects on scar tissue formation and further its potential as a base for skin tissue engineering.en_AU
dc.rightsThe author retains copyright of this thesis. It may only be used for the purposes of research and study. It must not be used for any other purposes and may not be transmitted or shared with others without prior permission.en_AU
dc.subjectcollagenen_AU
dc.subjectelastinen_AU
dc.subjecttemplateen_AU
dc.subjectscaffolden_AU
dc.subjectcross-linkingen_AU
dc.titleThe Next Step For A Collagen-Elastin Dermal Template In Skin Tissue Engineeringen_AU
dc.typeThesisen_AU
dc.type.thesisDoctor of Philosophyen_AU
usyd.facultySydney Medical Schoolen_AU
usyd.degreeMaster of Philosophy M.Philen_AU
usyd.awardinginstThe University of Sydneyen_AU


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