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dc.contributor.authorZhang, Jianping
dc.date.accessioned2019-04-09
dc.date.issued2018-12-18
dc.identifier.urihttp://hdl.handle.net/2123/20274
dc.description.abstractWheat is one of the first domesticated and cultivated crops together with barley. Rust diseases are considered as one of the major threats to wheat production in almost all of the wheat growing areas worldwide and disease control relies mainly on the utilisation of genetic resistance. The general objective of this doctoral study is to reduce the risk of yield loss due to rust diseases by providing genetic knowledge on wheat-rust pathosystems at the molecular level. In this thesis, integrated efforts have been devoted to i: understand rust pathogen populations at the molecular level; ii: characterise genetic resistance in the host; iii: understand the functional mechanisms underlying the plant-microbial interaction; and iv: to inform the effective deployment of characterised genetic resistance. In Chapter 3, the relationship between virulence and molecular patterns using SSR markers on selected isolates of Puccinia graminis f. sp. tritici (Pgt) collected in Australia during a 52 year period was examined and an evolutionary pathway involving these isolates was proposed. In Chapter 4 and 5, I focused on the stem rust resistance locus Sr26, derived from Thinopyrum ponticum (tall wheat grass), as an excellent example of the successful utilisation of genetic resistance from a wild relative of a crop plant that remains one of the few durable sources of resistance currently effective against all known field isolates of Pgt. I undertook the cloning of Sr26 by MutRenSeq pipeline. A candidate gene of Sr26 was successfully identified and I demonstrated that this gene candidate encoded a CNL (CC-NB-ARC-LRR) type of R protein. In Chapter 6, candidate genes were successfully identified for the stripe rust resistance genes Yr7, Yr5, and YrSP. Chapter 7 comprised the addendum of Chapter 6, the complementary transgenic validation for Yr5 and YrSP were provided.en_AU
dc.publisherUniversity of Sydneyen_AU
dc.publisherFaculty of Scienceen_AU
dc.publisherSchool of Life and Environmental Sciencesen_AU
dc.rightsThe author retains copyright of this thesis. It may only be used for the purposes of research and study. It must not be used for any other purposes and may not be transmitted or shared with others without prior permission.en_AU
dc.subjectWheaten_AU
dc.subjectRust diseaseen_AU
dc.subjectGeneticsen_AU
dc.subjectImmune receptorsen_AU
dc.subject.otherincludes published articlesen_AU
dc.titleStudies of the Wheat-rust Disease Pathosystem with a Focus on Resistance Gene Characterisationen_AU
dc.typePhD Doctorateen_AU
dc.type.pubtypeDoctor of Philosophy Ph.D.en_AU
dc.description.embargo2020-10-09


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