Regenerative medicine is an emerging field that aims to treat injury and disease by harnessing and augmenting the body’s innate capacity for tissue regeneration. Many of the strategies developed in this field have relied extensively on the principles of tissue engineering, a set of methods that bring together cells, cellular signals and material scaffolds to repair or replace biological tissue. While the number of novel tissue engineering strategies continues to rapidly expand, the innovations underlying these solutions often fail to consider the key technical, manufacturing, and regulatory barriers that prohibit these technologies from suitable use in humans. As a result, the field of tissue engineering has one of the lowest rates of clinical translation amongst medical research. To address this, this thesis examines each of the prominent components of the tissue engineering practice and develops tools and strategies that enable the development of solutions with high translational potential. The collective findings of these works propose tools and solutions applicable within the major facets of tissue engineering that may help to lay the groundwork for future therapies with high clinical probability in a number of regenerative medicine applications.