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|Title: ||Quality Assessment and Biological Activities of Centella Species in Diabetic Wound Healing|
|Authors: ||Alqahtani, Ali Saeed|
|Issue Date: ||30-Jun-2015|
|Publisher: ||University of Sydney|
Faculty of Pharmacy
|Abstract: ||The aim of this study was to differentiate Australian Centella species using morphological, genetic and phytochemical methods, and to evaluate their antiglycation and cell protection activities in relation to diabetic wound healing. The morphometric multivariate analysis successfully grouped the samples into three clusters: C. asiatica, C. cordifolia and C. erecta. Inter Simple Sequence Repeat (ISSR) genetic markers classified the samples according to the three species where C. erecta was the most genetically distinct. Multivariate chemometrics of HPTLC profiles of saponins and phenolics separated C. erecta from other two species. The total triterpene and phenolic content as well as chlorogenic acid were higher in C. cordifolia and lower in C. erecta. Ethyl acetate fraction of C. cordifolia exerted the most potent antioxidant and antiglycation activities. It ameliorated the MGO-induced inhibition of endothelial cells, fibroblasts and keratinocytes adhesion on extracellular matrix proteins. The results indicate that ISSR molecular techniques combined with morphological examination and phytochemical analysis are effective for quality assessment and systematic studies of Centella. These methods have enabled the differentiation of three Centella species in Australia. C. cordifolia is a potential candidate for further development as a medicinal product for diabetic wound healing.|
|Access Level: ||Access is restricted to staff and students of the University of Sydney . UniKey credentials are required. Non university access may be obtained by visiting the University of Sydney Library.|
|Rights and Permissions: ||The author retains copyright of this thesis. It may only be used for the purposes of research and study. It must not be used for any other purposes and may not be transmitted or shared with others without prior permission.|
|Type of Work: ||PhD Doctorate|
|Type of Publication: ||Doctor of Philosophy Ph.D.|
|Appears in Collections:||Sydney Digital Theses (University of Sydney Access only)|
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|ALQAHTANI Ali - Final Thesis.pdf||Thesis||5.31 MB||Adobe PDF|
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