Please use this identifier to cite or link to this item:
|Title: ||Development, characterization and pulmonary disposition of liposomal ciprofloxacin formulations|
|Authors: ||Cipolla, David Carter|
|Issue Date: ||12-Feb-2015|
|Publisher: ||University of Sydney|
Faculty of Pharmacy
|Abstract: ||The objective of this research was to develop and characterize novel liposomal ciprofloxacin formulations with modified release profiles, providing both faster and slower release of drug compared to the traditional liposomal formulation. The challenge was to do this without utilizing new lipid compositions, but instead by developing simple procedures to alter specific features of the liposomes. The new formulations were characterized by cryo-TEM analysis, dynamic light scattering, in vitro release assay, aerosol particle size distribution and the effect of mesh nebulization on functionality. A liposomal ciprofloxacin formulation was modified in two ways: 1. by addition of surfactant (e.g., 0.2% polysorbate 80 or 0.4% polysorbate 20) after osmotic swelling in a hypotonic environment to create a more permeable membrane which resulted in significantly faster release properties, and 2. by freeze-thaw to transform the drug into a nanocrystalline state inside the liposome to create a slower releasing formulation. For both situations, the liposomes maintained their vesicular structure. These experiments demonstrate two simple but novel ways to modify the encapsulation state and release properties of a liposome formulation. These examples demonstrate the potential to develop personalized therapies for patients by dialing-in the appropriate release properties of a liposomal drug product based on patient-based metrics.|
|Access Level: ||Access is restricted to staff and students of the University of Sydney . UniKey credentials are required. Non university access may be obtained by visiting the University of Sydney Library.|
|Rights and Permissions: ||The author retains copyright of this thesis. It may only be used for the purposes of research and study. It must not be used for any other purposes and may not be transmitted or shared with others without prior permission.|
|Type of Work: ||PhD Doctorate|
|Type of Publication: ||Doctor of Philosophy Ph.D.|
|Appears in Collections:||Sydney Digital Theses (University of Sydney Access only)|
This work is protected by Copyright. All rights reserved. Access to this work is provided for the purposes of personal research and study. Except where permitted under the Copyright Act 1968, this work must not be copied or communicated to others without the express permission of the copyright owner. Use the persistent URI in this record to enable others to access this work.
|CIPOLLA David - final thesis.pdf||Final Thesis||7.27 MB||Adobe PDF|
Items in Sydney eScholarship Repository are protected by copyright, with all rights reserved, unless otherwise indicated.