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|Title: ||Investigations into a new secretion pathway for the export of gingipain from Porphyromonas gingivalislis|
|Authors: ||Zhou, Xiaoyan|
|Issue Date: ||3-Feb-2015|
|Publisher: ||University of Sydney|
Faculty of Dentistry
|Abstract: ||Type IX Secretion System (T9SS) is a novel protein secretion pathway for numerous virulence factors in Porphyromonas gingivalis–the “keystone” pathogen in chronic periodontitis. The known substrates of this secretion system, including the major virulence factor, gingipains, carry a conserved C-terminal domain (CTD) which has been found to be cleaved during the translocation process and function as a recognition signal for the T9SS. However, multiple alignment of various CTDs failed to define a consensus sequence at the putative CTD processing site. In this doctoral candidature, we carried out site-directed mutagenesis and subtitutional manipulation of the β-hairpin fold of the Ig-like subdomain (IgLD), to show that the CTD recognition and cleavage at the site was dependent on the junction conformation between the CTD and adjecent IgLD instead of a specific residue or local sequence. Further, a novel CTD protein PG1604 was identified to be essential for maturation and secretion of gingipain and shown to be a new component of T9SS. Investigation into the secretion mechanism and novel components identification in this study may broaden our insights to this secretion system and provide opportunities to control the maturation of virulence factors from P. gingivalis in the treatment of periodontal disease.|
|Access Level: ||Access is restricted to staff and students of the University of Sydney . UniKey credentials are required. Non university access may be obtained by visiting the University of Sydney Library.|
|Rights and Permissions: ||The author retains copyright of this thesis. It may only be used for the purposes of research and study. It must not be used for any other purposes and may not be transmitted or shared with others without prior permission.|
|Type of Work: ||PhD Doctorate|
|Type of Publication: ||Doctor of Philosophy Ph.D.|
|Appears in Collections:||Sydney Digital Theses (University of Sydney Access only)|
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|ZHOU Xiaoyan - Final Thesis.pdf||Final Thesis||12.04 MB||Adobe PDF|
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