|Title:||MicroRNA Biomarkers in the Management of Papillary Thyroid Cancer|
|Authors:||Lee, James Cheng-Yen|
|Publisher:||University of Sydney.|
Kolling Institute of Medical Research.
Northern Clinical School.
|Abstract:||Papillary thyroid cancer (PTC) is a disease with a moderate recurrence rate, despite a low mortality rate. A cornerstone of PTC management is morbidity minimisation. MicroRNAs (miRNAs) are a class of RNAs showing great potential as diagnostic and prognostic biomarkers. This thesis explores the application of miRNAs as biomarkers of aggressive PTC biology and recurrence. Through miRNA microarrays and targeted validations, it was demonstrated that tumoral miR-193b was associated with central lymph node and distant metastasis, while miR-146b and miR-222 were associated with recurrence. Incorporation of these tissue biomarkers into the risk stratification algorithm at the initial treatment of PTC has the potential to guide the extent of required surgery, the level of adjuvant therapy and the intensity of long-term surveillance. Furthermore, circulating levels of miR-146b, miR-221, and miR-222 correlated with the presence of PTC. Secretion of miR-146b and miR-222 by PTC cells was further demonstrated in an in vitro model. These findings suggest that miRNAs are potential adjuncts to serum thyroglobulin as a non-invasive recurrence surveillance tool. Based on the findings of this thesis, minimisation of the morbidity in PTC patients can potentially be achieved through personalised treatment regimes utilising miRNA-guided risk stratification, and recurrence surveillance with circulating miRNAs.|
|Access Level:||Access is restricted to staff and students of the University of Sydney . UniKey credentials are required. Non university access may be obtained by visiting the University of Sydney Library.|
|Rights and Permissions:||The author retains copyright of this thesis. It may only be used for the purposes of research and study. It must not be used for any other purposes and may not be transmitted or shared with others without prior permission.|
|Type of Work:||PhD Doctorate|
|Type of Publication:||Doctor of Philosophy Ph.D.|
|Appears in Collections:||Sydney Digital Theses (University of Sydney Access only)|
|LEE James - Final Thesis.pdf||Thesis||17.14 MB||Adobe PDF|
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