|Title:||Modulating protein adsoption of calcium phosphate-based biomaterials|
|Authors:||Lee, Wing Hin|
|Publisher:||Faculty of Pharmacy.|
University of Sydney.
|Abstract:||The focus in this thesis was to examine the factors that influence protein adsorption onto hydroxyapatite (HA) surfaces and utilize these factors to improve HA properties to be used as a carrier for the delivery of bone growth factors in body. The ultimate aim was to prepare HA particles equipped with a high loading capacity for bone morphogenetic protein 2 (BMP-2), while simultaneously provide a sustained release of BMP-2 in the body. used widely as a bone substitute. The experimental approach focused on the methods used to selectively modify the surface charge of HA via immobilization of charged biological molecules such as amino acids and citric acid. The protein adsorption studies were investigated using two model proteins: basic protein (lysozyme) and acidic protein (BSA). In addition, manipulation of microenvironments (i.e. ionic strength, pH, temperature) was employed to achieve higher protein loading capacity and simultaneously to promote osteoblast attachment and differentiation. The understanding of the factors involved in protein adsorption could provide us with a better understating of strategies to manipulate protein adsorption onto HA, enabling us to further develop a superior HA biomaterial as a carrier for delivering therapeutic proteins (e.g., growth factors) in the body.|
|Access Level:||Access is restricted to staff and students of the University of Sydney . UniKey credentials are required. Non university access may be obtained by visiting the University of Sydney Library.|
|Rights and Permissions:||The author retains copyright of this thesis. It may only be used for the purposes of research and study. It must not be used for any other purposes and may not be transmitted or shared with others without prior permission.|
|Type of Work:||PhD Doctorate|
|Type of Publication:||Doctor of Philosophy Ph.D.|
|Appears in Collections:||Sydney Digital Theses (University of Sydney Access only)|
|LEE Wing Hin - Final Thesis.pdf||Thesis||56.36 MB||Adobe PDF|
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