• Extracellular allosteric Na+ binding to the Na+,K+-ATPase in cardiac myocytes 

      Garcia, Alvaro; Fry, Natasha A. S.; Karimi, Keyvan; Liu, Chia-chi; Apell, Hans-Jurgen; Rasmussen, Helge H.; Clarke, Ronald J.
      Published 2013-12-01
      Whole cell patch clamp measurements of the current, Ip, produced by the Na+,K+-ATPase across the plasma membrane of rabbit cardiac myocytes show an increase in Ip over the extracellular Na+ concentration range 0 – 50 mM. ...
      Open Access
      Article
    • Glutathionylation-dependence of N+-K+-pump currents can mimic reduced subsarcolemmal Na+ diffusion 

      Garcia, Alvaro; Liu, Chia-Chi; Cornelius, Flemming; Clarke, Ronald J.; Rasmussen, Helge H.
      Published 2016-03-01
      The existence of a subsarcolemmal space with restricted diffusion for Na+ in cardiac myocytes has been inferred from a transient peak electrogenic Na+-K+ pump current beyond steady state on re-exposure of myocytes to K+ ...
      Open Access
      Article
    • Identification of electric-field-dependent steps in the Na+,K+-pump cycle 

      Mares, Laura J.; Garcia, Alvaro; Rasmussen, Helge H.; Cornelius, Flemming; Mahmmoud, Yasser A.; Berlin, Joshua R.; Lev, Bogdan; Allen, Toby W.; Clarke, Ronald J.
      Published 2014-09-01
      The charge transporting activity of the Na+,K+-ATPase depends on its surrounding electric field. To isolate which steps of the enzyme’s reaction cycle involve charge movement we have investigated the response of the ...
      Open Access
      Article
    • Membrane accessibility of glutathione 

      Garcia, Alvaro; Eljack, Nasma D.; Sani, Marc-Antoine; Separovic, Frances; Rasmussen, Helge H.; Kopec, Wojciech; Khandelia, Himanshu; Cornelius, Flemming; Clarke, Ronald J.
      Published 2015-07-30
      Regulation of the ion pumping activity of the Na+,K+-ATPase is crucial to the survival of animal cells. Recent evidence has suggested that the activity of the enzyme could be controlled by glutathionylation of cysteine ...
      Open Access
      Article
    • Quantitative calculation of the role of the Na+,K+-ATPase in thermogenesis 

      Clarke, Ronald J.; Catauro, Michelina; Rasmussen, Helge H.; Apell, Hans-Jurgen
      Published 2013-07-11
      The Na+,K+-ATPase is accepted as an important source of heat generation (thermogenesis) in animals. Based on information gained on the kinetics of the enzyme’s partial reactions we consider via computer simulation whether ...
      Open Access
      Article
    • Redox-dependent regulation of the Na+-K+ pump: New twists to an old target for treatment of heart failure 

      Liu, Chia-Chi; Fry, Natasha A. S.; Hamilton, Elisha J.; Chia, Karin K. M.; Garcia, Alvaro; Galougahi, Keyvan K.; Figtree, Gemma A.; Clarke, Ronald J.; Bundgaard, Henning; Rasmussen, Helge H.
      Published 2013-05-30
      By the time it was appreciated that the positive inotropic effect of cardiac glycosides is due to inhibition of the membrane Na+-K+ pump, glycosides had been used for treatment of heart failure on an empiric basis for ~200 ...
      Open Access
      Article