Browsing by author "Garcia, Alvaro"
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Cholesterol depletion inhibits Na+,K+-ATPase activity in a near-native membrane environment
Garcia, Alvaro; Lev, Bogdan; Hossain, Khondker R.; Gorman, Amy; Diaz, Dil; Pham, T. H. Nguyen; Cornelius, Flemming; Allen, Toby W.; Clarke, Ronald J.Published 2019-02-15Cholesterol’s effects on Na+,K+-ATPase reconstituted in phospholipid vesicles have been extensively studied. However, previous studies have reported both cholesterol-mediated stimulation and inhibition of Na+,K+-ATPase ...Open AccessArticle -
Effect of posttranslational modification on the Na+, K+ ATPase kinetics
Garcia, AlvaroPublished 2014-08-29The Na+, K+ ATPase is an essential membrane protein in eukaryotic cells, which transports Na+ out of the cell in exchange for K+ into the cell. For this transport it hydrolyses one molecule of ATP for each cycle. The ...Open AccessThesis -
Electrostatic stabilization plays a central role in autoinhibitory regulation of the Na+,K+-ATPase
Jiang, Qiucen; Garcia, Alvaro; Han, Minwoo; Cornelius, Flemming; Apell, Hans-Jurgen; Khandelia, Himanshu; Clarke, Ronald J.Published 2017-01-24The Na+,K+-ATPase is present in the plasma membrane of all animal cells. It plays a crucial role in maintaining the Na+ and K+ electrochemical potential gradients across the membrane, which are essential in numerous ...Open AccessArticle -
Evidence for ATP interaction with phosphatidylcholine bilayers
Garcia, Alvaro; Pochinda, Simon; Elgaard-Jorgensen, Paninnguaq N.; Khandelia, Himanshu; Clarke, Ronald J.Published 2019-07-10ATP is a fundamental intracellular molecule and is thought to diffuse freely throughout the cytosol. Evidence obtained from nucleoside-sensing sarcolemmal ion channels and red blood cells, however, suggest that ATP is ...Open AccessArticle -
Extracellular allosteric Na+ binding to the Na+,K+-ATPase in cardiac myocytes
Garcia, Alvaro; Fry, Natasha A. S.; Karimi, Keyvan; Liu, Chia-chi; Apell, Hans-Jurgen; Rasmussen, Helge H.; Clarke, Ronald J.Published 2013-12-01Whole cell patch clamp measurements of the current, Ip, produced by the Na+,K+-ATPase across the plasma membrane of rabbit cardiac myocytes show an increase in Ip over the extracellular Na+ concentration range 0 – 50 mM. ...Open AccessArticle -
Glutathionylation-dependence of N+-K+-pump currents can mimic reduced subsarcolemmal Na+ diffusion
Garcia, Alvaro; Liu, Chia-Chi; Cornelius, Flemming; Clarke, Ronald J.; Rasmussen, Helge H.Published 2016-03-01The existence of a subsarcolemmal space with restricted diffusion for Na+ in cardiac myocytes has been inferred from a transient peak electrogenic Na+-K+ pump current beyond steady state on re-exposure of myocytes to K+ ...Open AccessArticle -
Identification of electric-field-dependent steps in the Na+,K+-pump cycle
Mares, Laura J.; Garcia, Alvaro; Rasmussen, Helge H.; Cornelius, Flemming; Mahmmoud, Yasser A.; Berlin, Joshua R.; Lev, Bogdan; Allen, Toby W.; Clarke, Ronald J.Published 2014-09-01The charge transporting activity of the Na+,K+-ATPase depends on its surrounding electric field. To isolate which steps of the enzyme’s reaction cycle involve charge movement we have investigated the response of the ...Open AccessArticle -
Interaction of N-terminal peptide analogues of the Na+,K+-ATPase with membranes
Nguyen, Khoa; Garcia, Alvaro; Sani, Marc-Antoine; Diaz, Dil; Dubey, Vikas; Clayton, Daniel; Dal Poggetto, Giovanni; Cornelius, Flemming; Payne, Richard J.; Separovic, Frances; Khandelia, Himanshu; Clarke, Ronald J.Published 2018-03-06The Na+,K+-ATPase, which is present in the plasma membrane of all animal cells, plays a crucial role in maintaining the Na+ and K+ electrochemical potential gradients across the membrane. Recent studies have suggested that ...Open AccessArticle -
Membrane accessibility of glutathione
Garcia, Alvaro; Eljack, Nasma D.; Sani, Marc-Antoine; Separovic, Frances; Rasmussen, Helge H.; Kopec, Wojciech; Khandelia, Himanshu; Cornelius, Flemming; Clarke, Ronald J.Published 2015-07-30Regulation of the ion pumping activity of the Na+,K+-ATPase is crucial to the survival of animal cells. Recent evidence has suggested that the activity of the enzyme could be controlled by glutathionylation of cysteine ...Open AccessArticle -
Polar interactions play an important role in the energetics of the main phase transition of phosphatidylcholine membranes
Garcia, Alvaro; Zou, Haipei; Hossain, Khondker R.; Xu, Qikui Henry; Buda, Annabelle; Clarke, Ronald J.Published 2019-01-08Conformational changes of membrane proteins are accompanied by deformation in the surrounding lipid bilayer. To gain insight into the energetics of membrane deformation, the phase behavior of dimyristoylphosphatidylcholine ...Open AccessArticle -
Redox-dependent regulation of the Na+-K+ pump: New twists to an old target for treatment of heart failure
Liu, Chia-Chi; Fry, Natasha A. S.; Hamilton, Elisha J.; Chia, Karin K. M.; Garcia, Alvaro; Galougahi, Keyvan K.; Figtree, Gemma A.; Clarke, Ronald J.; Bundgaard, Henning; Rasmussen, Helge H.Published 2013-05-30By the time it was appreciated that the positive inotropic effect of cardiac glycosides is due to inhibition of the membrane Na+-K+ pump, glycosides had been used for treatment of heart failure on an empiric basis for ~200 ...Open AccessArticle -
Stimulation of Na+,K+-ATPase activity as a possible driving foce in cholesterol evolution
Lambropoulos, Nicholas; Garcia, Alvaro; Clarke, Ronald J.Published 2015-12-29Cholesterol is exclusively produced by animals and is present in the plasma membrane of all animal cells. In contrast, the membranes of fungi and plants contain other sterols. To explain the exclusive preference of animal ...Open AccessArticle -
The voltage-sensitive dye RH421 detects a Na+,K+-ATPase conformational change at the membrane interface
Garcia, Alvaro; Promod, Pratap R.; Lupfert, Christian; Cornelius, Flemming; Jacquemin, Denis; Lev, Bogdan; Allen, Toby W.; Clarke, Ronald J.Published 2017-01-20RH421 is a voltage-sensitive fluorescent styrylpyridinium dye which has often been used to probe the kinetics of Na+,K+-ATPase partial reactions. The origin of the dye’s response has up to now been unclear. Here we show ...Open AccessArticle
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