• Anion carriers as potential treatments for cystic fibrosis: transport in cystic fibrosis cells, and additivity to channel-targeting drugs† 

      Li, Hongyu; Valkenier, Hennie; Thorne, Abigail G.; Dias, Christopher M.; Cooper, James A.; Kieffer, Marion; Busschaert, Nathalie; Gale, Philip A.; Sheppard, David N.; Davis, Anthony P.
      Published 2019-10-01
      Defective anion transport is a hallmark of the genetic disease cystic fibrosis (CF). One approach to restore anion transport to CF cells utilises alternative pathways for transmembrane anion transport, including artificial ...
      Open Access
      Article
    • Dissecting the chloride–nitrate anion transport assay 

      Yang, Yufeng; Wu, Xin; Busschaert, Nathalie; Furuta, Hiroyuki; Gale, Philip A.
      Published 2017-07-01
      A systematic study of chloride vs. nitrate selectivity across six anion transporters has revealed a good correlation between the selectivities of their anion binding and membrane transport properties. This work reveals the ...
      Open Access
      Article
    • Fluorinated synthetic anion carriers: experimental and computational insights into transmembrane chloride transport 

      Spooner, Michael J.; Li, Hongyu; Marques, Igor; Costa, Pedro M.R.; Wu, Xin; Howe, Ethan N.W.; Busschaert, Nathalie; Moore, Stephen J.; Light, Mark E.; Sheppard, David N.; Gale, Philip A.
      Published 2018-01-01
      A series of fluorinated tripodal tris-thioureas function as highly active anion transporters across lipid bilayers and cell membranes. Here, we investigate their mechanism of action using anion transport assays in cells ...
      Open Access
      Article
    • Full elucidation of the transmembrane anion transport mechanism of squaramides using in silico investigations 

      Marques, Igor; Costa, Pedro M.R.; Miranda, Margarida Q.; Busschaert, Nathalie; Howe, Ethan N.W.; Clarke, Harriet J.; Haynes, Cally J.E.; Kirby, Isabelle L.; Rodilla, Ananda M.; Pérez-Tomás, Ricardo; Gale, Philip A.; Félix, Vítor
      Published 2018-01-01
      A comprehensive experimental and theoretical investigation of the transmembrane chloride transport promoted by four series of squaramide derivatives, with different degrees of fluorination, number of convergent N–H binding ...
      Open Access
      Article
    • Full elucidation of the transmembrane anion transport mechanism of squaramides using in silico investigations 

      Marques, Igor; Costa, Pedro M.R.; Miranda, Margarida Q.; Busschaert, Nathalie; Howe, Ethan N.W.; Clarke, Harriet J.; Haynes, Cally J.E.; Kirby, Isabelle L.; Rodilla, Ananda M.; Pérez-Tomás, Ricardo; Gale, Philip A.; Félix, Vítor
      Published 2018-06-29
      A comprehensive experimental and theoretical investigation of the transmembrane chloride transport promoted by four series of squaramide derivatives, with different degrees of fluorination, number of convergent N–H binding ...
      Open Access
      Article
    • Managing research throughout COVID-19: Lived experiences of supramolecular chemists 

      Leigh, Jennifer S; Hiscock, Jennifer R; Koops, Sarah; McConnell, Anna J; Haynes, Cally J E; Caltagirone, Claudia; Kieffer, Marion; Draper, Emily R; Slater, Anna G; Hutchins, Kristin M; Watkins, Davita; Busschaert, Nathalie; von Krbek, Larissa K S; Jolliffe, Katrina A; Hardie, Michaele J
      Published 2022
      The international Women in Supramolecular Chemistry network believes that taking an area-specific approach effectively supports equality, diversity, and inclusion. Science lacks diversity, and this is intersectional. We ...
      Article
    • New Insights into the Anion Transport Selectivity and Mechanism of Tren-based Tris-(thio)ureas 

      Jowett, Laura A.; Howe, Ethan N.W.; Wu, Xin; Busschaert, Nathalie; Gale, Philip A.
      Published 2018-06-19
      The anion transport properties of a series of previously reported tren-based anionophores have been revisited using new assays designed to measure anion uniport. This study provides new insights into the transport mechanism ...
      Open Access
      Article
    • Real-Time Recording of the Cellular Effects of the Anion Transporter Prodigiosin 

      Cheung, Shane; Wu, Dan; Daly, Harrison C.; Busschaert, Nathalie; Morgunova, Marina; Simpson, Jeremy C.; Scholz, Dimitri; Gale, Philip A.; O'Shea, Donal F.
      Published 2018-04-12
      The unraveling of the cellular effects of anion transporters is key to their potential development as apoptosis-inducing or autophagy-disrupting therapeutics. Here, we conducted a systematic study of the cellular responses ...
      Open Access
      Preprint
    • Squaramide-based synthetic chloride transporters activate TFEB but block autophagic flux 

      Zhang, Shaoyi; Wang, Yan; Xie, Wei; Howe, Ethan N.W.; Busschaert, Nathalie; Sauvat, Allan; Leduc, Marion; Gomes-da-Silva, Ligia C.; Chen, Guo; Martins, Isabelle; Deng, Xiaxing; Maiuri, Luigi; Kepp, Oliver; Soussi, Thierry; Gale, Philip A.; Zamzami, Naoufal; Kroemer, Guido
      Published 2019-02-01
      Cystic fibrosis is a disease caused by defective function of a chloride channel coupled to a blockade of autophagic flux. It has been proposed to use synthetic chloride transporters as pharmacological agents to compensate ...
      Open Access
      Article