Now showing items 1-8 of 8

    • Anion carriers as potential treatments for cystic fibrosis: transport in cystic fibrosis cells, and additivity to channel-targeting drugs† 

      Li, Hongyu; Valkenier, Hennie; Thorne, Abigail G.; Dias, Christopher M.; Cooper, James A.; Kieffer, Marion; Busschaert, Nathalie; Gale, Philip A.; Sheppard, David N.; Davis, Anthony P. (Royal Society of Chemistry, 2019-10)
      Defective anion transport is a hallmark of the genetic disease cystic fibrosis (CF). One approach to restore anion transport to CF cells utilises alternative pathways for transmembrane anion transport, including artificial ...
    • Dissecting the chloride–nitrate anion transport assay 

      Yang, Yufeng; Wu, Xin; Busschaert, Nathalie; Furuta, Hiroyuki; Gale, Philip A. (Royal Society of Chemistry, 2017-07)
      A systematic study of chloride vs. nitrate selectivity across six anion transporters has revealed a good correlation between the selectivities of their anion binding and membrane transport properties. This work reveals ...
    • Fluorinated synthetic anion carriers: experimental and computational insights into transmembrane chloride transport 

      Spooner, Michael J.; Li, Hongyu; Marques, Igor; Costa, Pedro M.R.; Wu, Xin; Howe, Ethan N.W.; Busschaert, Nathalie; Moore, Stephen J.; Light, Mark E.; Sheppard, David N.; Gale, Philip A. (Royal Society of Chemistry, 2018)
      A series of fluorinated tripodal tris-thioureas function as highly active anion transporters across lipid bilayers and cell membranes. Here, we investigate their mechanism of action using anion transport assays in cells ...
    • Full elucidation of the transmembrane anion transport mechanism of squaramides using in silico investigations 

      Marques, Igor; Costa, Pedro M.R.; Miranda, Margarida Q.; Busschaert, Nathalie; Howe, Ethan N.W.; Clarke, Harriet J.; Haynes, Cally J.E.; Kirby, Isabelle L.; Rodilla, Ananda M.; Pérez-Tomás, Ricardo; Gale, Philip A.; Félix, Vítor (Royal Society of Chemistry, 2018)
      A comprehensive experimental and theoretical investigation of the transmembrane chloride transport promoted by four series of squaramide derivatives, with different degrees of fluorination, number of convergent N–H binding ...
    • Full elucidation of the transmembrane anion transport mechanism of squaramides using in silico investigations 

      Marques, Igor; Costa, Pedro M.R.; Miranda, Margarida Q.; Busschaert, Nathalie; Howe, Ethan N.W.; Clarke, Harriet J.; Haynes, Cally J.E.; Kirby, Isabelle L.; Rodilla, Ananda M.; Pérez-Tomás, Ricardo; Gale, Philip A.; Félix, Vítor (Royal Society of Chemistry, 2018-06-29)
      A comprehensive experimental and theoretical investigation of the transmembrane chloride transport promoted by four series of squaramide derivatives, with different degrees of fluorination, number of convergent N–H binding ...
    • New Insights into the Anion Transport Selectivity and Mechanism of Tren-based Tris-(thio)ureas 

      Jowett, Laura A.; Howe, Ethan N.W.; Wu, Xin; Busschaert, Nathalie; Gale, Philip A. (Wiley VCH, 2018-06-19)
      The anion transport properties of a series of previously reported tren-based anionophores have been revisited using new assays designed to measure anion uniport. This study provides new insights into the transport ...
    • Real-Time Recording of the Cellular Effects of the Anion Transporter Prodigiosin 

      Cheung, Shane; Wu, Dan; Daly, Harrison C.; Busschaert, Nathalie; Morgunova, Marina; Simpson, Jeremy C.; Scholz, Dimitri; Gale, Philip A.; O'Shea, Donal F. (Cell Press, 2018-04-12)
      The unraveling of the cellular effects of anion transporters is key to their potential development as apoptosis-inducing or autophagy-disrupting therapeutics. Here, we conducted a systematic study of the cellular responses ...
    • Squaramide-based synthetic chloride transporters activate TFEB but block autophagic flux 

      Zhang, Shaoyi; Wang, Yan; Xie, Wei; Howe, Ethan N.W.; Busschaert, Nathalie; Sauvat, Allan; Leduc, Marion; Gomes-da-Silva, Ligia C.; Chen, Guo; Martins, Isabelle; Deng, Xiaxing; Maiuri, Luigi; Kepp, Oliver; Soussi, Thierry; Gale, Philip A.; Zamzami, Naoufal; Kroemer, Guido (Springer Nature, 2019-02)
      Cystic fibrosis is a disease caused by defective function of a chloride channel coupled to a blockade of autophagic flux. It has been proposed to use synthetic chloride transporters as pharmacological agents to compensate ...